end repaired cdna Search Results


t5 caption a7 reagent concentration amount ul end repaired cdna product  (New England Biolabs)
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New England Biolabs t5 caption a7 reagent concentration amount ul end repaired cdna product
T5 Caption A7 Reagent Concentration Amount Ul End Repaired Cdna Product, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext solid dna library preparation kit end repair module  (New England Biolabs)
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Nebnext Solid Dna Library Preparation Kit End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kapa dna library preparation kit for illumina sequencing  (Illumina Inc)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Kapa Dna Library Preparation Kit For Illumina Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra ii dna library prep kit  (New England Biolabs)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Nebnext Ultra Ii Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neb next ultra ii end repair a tailing dna library prep kit  (New England Biolabs)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Neb Next Ultra Ii End Repair A Tailing Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neb next ultra ii end repair a tailing dna library prep kit - by Bioz Stars, 2026-08
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dna library  (New England Biolabs)
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New England Biolabs dna library
fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Dna Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra ii rna library prep  (New England Biolabs)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Nebnext Ultra Ii Rna Library Prep, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra ii dna library prep kit for illumina  (Illumina Inc)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra ii dna library prep kit for illumina - by Bioz Stars, 2026-08
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t4 dna polymerase 3  (New England Biolabs)
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New England Biolabs t4 dna polymerase 3
fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
T4 Dna Polymerase 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext dna library prep kit  (New England Biolabs)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Nebnext Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/end+repaired+cdna/pmc06245762-225-13-18?v=New+England+Biolabs
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nebnext adaptor  (New England Biolabs)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Nebnext Adaptor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumina truseq kit  (Illumina Inc)
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fDNA enrichment results. (A) Percentage of <t>sequencing</t> reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial <t>DNA</t> in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).
Illumina Truseq Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fDNA enrichment results. (A) Percentage of sequencing reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial DNA in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).

Journal: Genetics

Article Title: Efficient Genome-Wide Sequencing and Low-Coverage Pedigree Analysis from Noninvasively Collected Samples

doi: 10.1534/genetics.116.187492

Figure Lengend Snippet: fDNA enrichment results. (A) Percentage of sequencing reads that mapped to the baboon genome and were not PCR duplicates (“Mapped,” dark blue), mapped and were PCR duplicates (“PCR Duplicate,” blue), or did not map and likely represent environmental or bacterial DNA in the case of fDNA/aDNA and unmappable fragments in the case of gDNA (“Other,” light blue). “gDNA” represents genomic DNA derived from the blood samples for LIT and HAP; “aDNA” represents ancient DNA data from capture-based enrichment reported in Carpenter et al. (2013 ). Numbers above each bar show the total number of PCR cycles used in each protocol. (B) Capture 2 produced significantly greater genome coverage than capture 1, despite a similar number of reads generated per sample (two-sample t -test, T = 9.7, P = 3.0 × 10 −12 ). On average in capture 2, we obtained ∼0.73× coverage of the genome with 5.76 Gb of sequencing. If all 5.76 Gb mapped to the baboon genome as non-PCR duplicates, we would have produced ∼2.2× genome-wide coverage. (C) Capture 2 also produced significantly greater fold enrichment of baboon DNA (fold enrichment is measured as percentage of nonduplicate baboon DNA postcapture divided by percentage of baboon DNA precapture: two-sample t -test, T = 4.4, P = 7.3 × 10 −5 ). (D) The amount of baboon DNA in the sample precapture [percentage of baboon DNA precapture, based on qPCR of the single-copy c-myc gene ( Morin et al. 2001 )] is strongly correlated with the percentage of baboon fragments obtained in postenrichment sequencing (Pearson’s r = 0.80, P = 1.0 × 10 −11 ). However, even samples with low amounts of endogenous DNA (<2%) exhibit substantial fold enrichment using our protocol (mean capture1 = 10.60×, mean capture2 = 24.82×).

Article Snippet: To generate baits, we sheared 5 μg of purified DNA to a mean fragment size of 150 bp and then end repaired and A-tailed the fragments, using the KAPA DNA Library Preparation Kit for Illumina Sequencing.

Techniques: Sequencing, Derivative Assay, Ancient DNA Assay, Produced, Generated, Genome Wide